Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter ADRB2 Total Internalization HEK 293 Cell Line, 2 vials. (93-0800C1)
The PathHunter ADRB2 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of ADRB2 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged ADRB2 and a ProLink-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the ADRB2 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC CX16-RV2 TZ; Cervix transformation zone; Human (Homo sapiens)
There is a Permit requirement: License form needed to be completed Material Transfer Agreement Addendum for Screening Application
The forms are available here: https://www.atcc.org/support/order-support/permits-and-restrictions
ATCC Streptococcus pyogenes; group a, type 38
Streptococcus pyogenes typing strain C94 is serotype 38 and is classified as Lancefield's group A. This aerobic bacterium can be used in emerging infectious disease research.
Eurofins Discoverx PathHunter CHO-K1 mCCR3 β-Arrestin Cell Line, 2 vials. (93-0522C2)
The PathHunter mCCR3 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CCL24) based activation of mCCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCCR3 and EA-tagged β-Arrestin-2. Activation of mCCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 mFPR1 β-Arrestin Cell Line, 2 vials. (93-0570C2)
The PathHunter mFPR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. formylpeptides) based activation of mFPR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mFPR1 and EA-tagged β-Arrestin-2. Activation of mFPR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC HCC1954 BL; Lymphoblast; Human (Homo sapiens)
HCC1954 BL is a B lymphoblast cell that was isolated from an 61-year-old, Asian, female's peripheral blood. The cell line can be used in cancer and immunology research.
Eurofins Discoverx PathHunter U2OS MC4R(T162I) ENDO-EA Pharmacotrafficking Assay, 2 vials. (93-1004C3)
The PathHunter U2OS MC4R(T162I) Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting MC4R. This cell line enables assessment of chaperone-based stabilization of MC4R(T162I) via detection of rescued membrane-endosome trafficking. The included cell line overexpresses ProLink-tagged mutant MC4R(T162I), which is misfolded and retained in the ER. The cell line also expresses endosome-localized EA. When a pharmacochaperone stabilizes the mutant MC4R, it is trafficked to the membrane and then internalized to the endosome, producing EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter U2OS mFFAR1 β-Arrestin Cell Line, 2 vials. (93-0975C3)
The PathHunter mFFAR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of mFFAR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mFFAR1 and EA-tagged β-Arrestin-2. Activation of mFFAR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 RXFP2 Gs Cell Line, 2 vials. (95-0140C2)
The cAMP Hunter RXFP2 (LGR8) Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. relaxin-1) based activation of RXFP2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type RXFP2, a naturally Gs coupled GPCR. Upon activation of RXFP2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel V, 8 tissue spots, 5 slides/PK
BioChain's FFPE multiple tissue panels with different types of tumors are designed for screening gene expression in multiple tumor tissues. Each tumor tissue is paired with a corresponding normal control tissue. A licensed pathologist issued a pathological diagnosis of each tumor based on examination of tissue sections. Documentation of tissues' clinical histories is available upon request. mRNA Northern blot and Western blots as well as total RNA Norterhn blot are available in identical formats.
Eurofins Discoverx PathHunter U2OS EphA7 Functional Assay, 2 vials. (93-0841C3)
The PathHunter U2OS EphA7 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated EphA7 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged EphA7 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Amsbio LLC Lyophilized microvesicles from NCI-H1975 cell line
Lyophilized microvesicles from NCI-H1975 cell line
Biochain Institute Inc Frozen Tissue Section - Progressive Supranuclear Palsy: Brain: Pons, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.